From: S.P. Monard (spm1007@cus.cam.ac.uk)
Date: Mon Jun 26 1995 - 04:41:35 EST
On Fri, 23 Jun 1995, Kjartan Egeberg wrote: > I intend to use fluorogenic beta-galactosidase substrate to study gene > expression in SW480-cells that are stable transfected with the gene encoding > beta-gal (reporter-gene)..... We have been using the FluoReporter kit from Molecular Probes part number F-1930 (50 test kit). We find it works pretty well. No fixation is required and the cells can easily be sorted and even grown. Transfected cells are usually about a log up from the control or untransfected cells. You must however keep the sample cold as untransfected cells quite rapidly convert the substrate at room temperature. We havn't co-labelled with antibodies but I don't see why this should be a problem. Good luck Simon Monard Cambridge UK
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