From: Jason, Janine (jmj1@CIDHIV7.EM.CDC.GOV)
Date: Fri Jun 09 1995 - 15:46:00 EST
To people doing intracellular (directly conjugated) and surface staining(also directly conjugated): Do you find you have flourescent signal shifts doing the above, vs doing all surface staining (3-color)? If so, do you have any idea why it happens? If not, could you share with me your protocol for intracellular staining? Many thanks, Janine Jason
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