From: Alan Bishop (ABISHOP@immuno.imvs.sa.gov.au)
Date: Wed May 24 1995 - 14:45:57 EST
I am posting this on behalf of Joy Mundy who works in the IMVS Immuno-Haematology Diagnostic Laboratory. ------------------------------------------------------------------ We type acute leukaemia patients, at presentation, for a number of markers; CD7,CD10,CD19,CD13,CD33 and CD34. Currently we stain for single markers only with these antibodies, on ficoll separated cells. We would like to perform dual and tri colour analysis on whole bone marrow. We wish to identify a unique cell marker combination on the malignant cell population for each patient. Once established we would like to use this unique set of markers to look for residual disease or relapse cells in marrow. Any ideas on a good protocol? +-------------------------------------------------+ | | | Alan Bishop | | Flow Cytometry Laboratory | | Hanson Centre for Cancer Research | | Institute of Medical and Veterinary Science | | PO Box 14 Rundle Mall | | Adelaide SA 5000 | | AUSTRALIA | | | | Voice : 61-8-228 7258 | | Fax : 61-8-232 4092 | | Email : bishop@immuno.imvs.gov.sa.au | | | +-------------------------------------------------+
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