Re: Green Fluorescent Protein

From: Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
Date: Thu Apr 27 1995 - 16:44:14 EST

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---Kier Watkins wrote:
 Someone has asked me about using Green Fluorescent Protein for flow analysis
of transfected sperm. 
What's the excitation/emission?
--- end of quoted material ---

I'm not sure whether the puns are intentional or not.... but I'll pretend I
didn't notice them. There is a paper in Science 263:802(1994) by Chalfie et al
that shows GFP spectra.  Excitation has a peak at 395nm with a shoulder at
470nm  and fluorescence has a peak at 509nm with a shoulder at 540nm.

Alice Givan
Englert Cell Analysis Laboaratory
Dartmouth Medical School
Lebanon,  NH 03756
USA

• Next message: kweber@vs4.im.med.umich.edu: "simulateous antigens plus DNA"
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• Next in thread: Doug Terle: "Re: Green Fluorescent Protein"
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