From: P.Openshaw Medicine (p.openshaw@ic.ac.uk)
Date: Mon Apr 24 1995 - 08:40:24 EST
In response to Clive Gray: > We are trying a saponin permeabilization technique - to measure > intracytoplasmic cytokines and other proteins (p24 etc). We are > initially fixing with 4% formaldehyde and then coming in with saponin > and then labelling with our Ab. Final conc 4% or 2%? Our protocol uses equal volumes of cells and 4%, i.e. 2% final, slightly hypertonic. Duration of fix and temperature can also be critical. ******************************************************************* *Peter Openshaw p.openshaw@ic.ac.uk * *Respiratory Medicine Tel:+44 171 723 1252 x 5781/6 * *St. Mary's Hosp. Med. School Fax:+44 171 724 7349 * *Imperial College, Norfolk Place, London W2 1PG, UK *
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