Re: removal of IgG from fc receptors

From: * (@VM.CC.PURDUE.EDU:HADAM@DHVMHH1)
Date: Mon Apr 10 1995 - 18:33:32 EST

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Another way to strip cells very efficiently from IgG is hypotonic
treatment. Take care to keep everything ice-cold and restore
isotonicity after 30 sec. Do not extend hypotonic conditions any
longer (this _is_ critical; use a stopwatch). Wash once with
ice-cold medium.
We used this procedure way back when Fc-receptors where still
quantitated using EA-rosettes.

Hope this helps-

Martin R. Hadam

Dr. Martin R. Hadam
Medizinische Hochschule Hannover
Kinderklinik
D-30623 HANNOVER
TEL (49) 511 532 9009                E-MAIL HADAM@DHVMHH1.BITNET
FAX (49) 511 532 9016                       HADAM@VM.MHRZ.MH-HANNOVER.DE
Bundesrepublik Deutschland

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