From: Eric Martz (emartz@microbio.umass.edu)
Date: Thu Apr 06 1995 - 19:19:00 EST
In message Thu, 06 Apr 1995 12:23:24 +1100, J.Webster@centenary.usyd.edu.au (Joseph Webster) writes: > PROBLEM: Profile data does not appear to contain any equivalent to the FCS > "$TOT" keyword; That means that all the analysis software at my disposal > cannot analyse the files, because they appear to contain NO events! You're right, Pro2FCS files do not provide $TOT (in any FCS form). I've modified MFI to work fine with them, though. Here's an excerpt from a beta-test announcement for MFI I made on 1/13/95. (Subsequently, MFI 3.4I has been released including these features.) [BEGIN QUOTE] A peculiarity of this [Pro2FCS] file structure is the absence of $TOT. I calculate the total number of events from the parameter data sizes and the offsets. If anyone is interested, ask and I can make available the C code I use. I do this for all files anyway since in some cases the number of events actually recorded in the data block is less than that given in $TOT. [A warning message is emitted when there's a discrepancy.] I have not been able to obtain a Pro2FCS file containing TIME as a parameter so MFI may not interpret the TIME range properly when it first encounters such a file. [END QUOTE] I still have never obtained a Pro2FCS file with TIME. If someone has such, please put a sample in /incoming at flowcyt.bio.umass.edu for my and MFI's edification. /*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Eric Martz, Professor of Immunology emartz@microbio.umass.edu Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578 Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/
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