From: Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
Date: Thu Feb 02 1995 - 16:45:30 EST
Read an article in a recent issue of Cytometry (17:294) by Amersfoort and Strijp -- about how different brands of Trypan Blue quench more or less efficiently (Merck was best). This explained to me why I wasn't able to quench FITC fluorescence well -- even with Trypan blue as thick as sludge. We did have good luck quenching with an anti-FITC antibody from Molecular Probes (an expensive way to go, if Merck TB would do just as well....). Haven't tried it with beads. Alice Givan Englert Cell Analysis Laboratory Dartmouth Medical School Lebanon, NH, USA tel 603-650-7907
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