RBC counts in Turkeys

From: ABBOTT DAVID L JR (ABBOTT_DAVID_L_JR@Lilly.com)
Date: Mon Jan 23 1995 - 14:34:00 EST

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One of our scientists is interested in using flow cytometry to determine 
absolute RBC counts in turkeys (the feathered kind). Our routine 
hematology instruments will not differentiate the nucleated RBCs. A 
FACScan and VANTAGE (soon) are available for the assay, but I would 
prefer to use the former.

Are any pan leukocyte markers available for turkeys? Will the RBCs stain 
differentially with DNA dyes? Has anyone used the Cytek absolute count 
system with a FACScan?

To start with, I am thinking of the following experiment.

1. Fix peripheral blood with 1% glutaraldehyde/PBS, at 4 degrees C 
overnight. 

2. Aspirate fixative and resuspend in PBS with 30 Ug/ml SDS to sphere RBCs.

3. Run samples and look for light scatter clusters.

Am I anywhere near the ball park here? Thanks for your comments.

Regards,

David Abbott
DL.Abbott@Lilly.Com

P.S. My ISAC directory internet address is wrong. Please substitute a 
"L" for the "K".
 

      

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