From: Ron Hoebe (hoebe@AMC.UVA.NL)
Date: Thu Dec 15 1994 - 04:42:16 EST
>To: BICH@PATD01.HS.SLL.SE (Birger Christensson)
>From: hoebe@amc.uva.nl (Ron Hoebe)
>Subject: Re: MALARIA INFECTED CELLS
>
>>IS THERE ANYONE WITH EXPERIENCE OF USING A FACSCAN FOR QUANTITATING MALARIA
>>INFECTED ERYTHROCYTES. OUR INITIAL EXPERIMENTS INDICATE THAT PI CAN BE USED
>>AFTER GLUTARALDEHYDE FIXATION AND TWEEN PERMEABILIZATION. HOWEVER. I GUESS
>>THERE MAY BE BETTER PROTOCOLS USING MEMBRANE PARMEABLE DYES?
>>ALL SUGGSETIONS ARE WELLCOME
>>
>>-------------------------------------------------------------------------
>> Birger Christensson, MD, PhD
>> Dept. of Pathology, F49
>> Huddinge University Hospital,
>> S-14186 Huddinge,
>> SWEDEN,
>> Tel +46-8-7461000
>> Fax +46-8-7795520
>> bich@PATD01.HS.SLL.SE
>>
>>
>>
>>
Dear Birger Christensson,
We have done it on a FACSTAR+.
We used 20 ul Hoechst 33342 (MEMBRANE PARMEABLE DYE) for 2 ml cell
suspension. ncubate for 1 our, 37 Celsius in the Dark. You have to exitate
with uv.
I understand that you have a FACSCAN so you don't have uv.
There is a new dye called YOYO 1 from Becton Dickenson. For more information
about this you should contact:
Maarten van de Keur
Academic Hospital Leiden CKHL
tel: +31 71 261783
or: +31 71 276831
Sincirely, Ron.
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