From: Dr Mike Salmon (salmonm@rheuma.bham.ac.uk)
Date: Mon Nov 21 1994 - 10:09:25 EST
Date: 17 Nov 94 19:16:58 EST From: Alice.L.Givan@Dartmouth.EDU (Alice L. Givan) Subject: anti-CD4 antibodies To: cytometry@flowcyt.cyto.purdue.edu I have been told that there are two types of anti-CD4 mAbs: those that bind to an epitope proximal to the T-cell surface and those that bind to a more distal epitope. As I am looking for an antibody that will bind to a site that is resistant to enzyme digestion, I would like to try both classes of mAb. Does anyone on the flow network have any information about these two types of anti-CD4 Abs -- and how I can find out which commercial antibodies fall into which category? Thanks. Alice, I haven't played with this for a few years, but the best paper on the subject I can remember which should help you is as follows: FUNCTIONAL EPITOPE ANALYSIS OF THE HUMAN CD4 MOLECULE - THE MHC CLASS-II-DEPENDENT ACTIVATION OF RESTING T-CELLS IS INHIBITED BY MONOCLONAL-ANTIBODIES TO CD4 REGARDLESS WHETHER OR NOT THEY RECOGNIZE EPITOPES INVOLVED IN THE BINDING OF MHC CLASS-II OR HIV GP120 MERKENSCHLAGER_M, BUCK_D, BEVERLEY_PCL, SATTENTAU_QJ JOURNAL OF IMMUNOLOGY 1990 Vol.145 No.9 pp.2839-2845 Have fun, Mike Salmon ............................ Mike Salmon Dept of Rheumatology The University of Birmingham Birmingham B15 2TT United Kingdom Tel 44 (0)21 414 6780 Fax 44 (0)21 414 6794
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