From: Dave Coder (dave@nucleus.immunol.washington.edu)
Date: Tue Nov 15 1994 - 14:43:31 EST
Although the normal mode of infection for T. cruzi is via a bug bite, the trypanasome will easily cross mucus membrane (infection via the conjunctiva is common). How much of an aerosol will sorting create? How many viable trypanasomes will be floating around? You should be able to have a well contained sorting area (negative pressure, properly shielded from the outside, etc.) BUT, there is no cure for T. cruzi infection: once infected you have a parasite for life. So, do you need to sort with a flow cytometer? I'd look at the alternatives first. Dave Coder Univ. Washington dcoder@u.washington.edu Begin forwarded message: X-Popmail-Charset: English Date: Mon, 14 Nov 94 20:48:26 EST From: (Steve G. Hilliard) <hilliard@zookeeper.zoo.uga.edu> To: cytometry@flowcyt.cyto.purdue.edu Subject: Sorting Biohazards--second try I've had trouble "following my bouncing mail" (can't sing anyway ;-)) but I get the feeling my first attempt didn't make it. To reiterate, I have a user who wants me to SORT antibody-labelled, VIABLE _T. cruzi_. I'd appreciate anyone's thoughts--current regulations (if any), precautions, "don't even consider it", etc. Do we need to put our Elite in a P2 room, or just forget it entirely? Anyone? Thanks, Steve ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Steve G. Hilliard, Cell Analysis Facility | University of Georgia | "Be good and you will hilliard@zookeeper.zoo.uga.edu | be lonesome..." ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
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