cytokine flow cytometry

From: Calman Prussin (Calman_Prussin@d10.niaid.pc.niaid.nih.gov)
Date: Wed Nov 09 1994 - 19:43:33 EST

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I missed ISAC, but heard there were some data shown demonstrating 
intracellular staining for cytokines.

If anyone saw this and would like to comment on:

1. Cytokines detected. IL-4,IL-5 or IL-10?

2. Cells examined. PBMC or ?

3. Methods. The usual PFA and saponin?

4. What was used to stimulate the cells.

4. Quality of data. Did it look believable? What were the controls. How 
much noise and nonspecific binding? 

                     Thanks,


                     Calman Prussin
                     Allergic Diseases Section
                     National Institute of Allergy and Infectious Diseases 
                     National Institutes of Health
                     Bethesda, Maryland    U.S.A.

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