antiPCNA-fitc

From: Rochelle A. Diamond (diamond@cco.caltech.edu)
Date: Fri Nov 04 1994 - 12:56:51 EST

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I am posting this for a friend who does not have an infobahn onramp in 
her vicinity.  So please respond back to me and I will relay the info.
Thanks.

Rochelle Diamond
Caltech Flow Cytometry Facility
diamond@cco.caltech.edu

Does anyone have any experience using DAKO's anti-PCNA-fitc in rat liver 
cells?  I am experiencing difficulty with inappropriate G1 staining in 
these cells.  We are using triton x-100 to wash out free antibody.  This 
system seems to work in human cells but upon switching to rat liver cells 
there is a problem.

Any help or suggestions would gratefully be accepted.

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