Re: Anti-TCR cross-reactivity?

From: Martin R. Hadam (@VM.CC.PURDUE.EDU:HADAM@DHVMHH1)
Date: Wed Oct 26 1994 - 14:18:34 EST

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In reply to: Roger Burger: "Anti-TCR cross-reactivity?"
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Interesting (and annoying) problem. I have some questions

(1) On whole blood, did the RBC stain ? (I wasn't shure if I got that right)
(2) Does this happen with samples from different donors and/or with different
    TCR (alpha/beta) antibodies ?
(3) What happens if you stain isolated (enriched) RBC e.g. from a ficoll pellet
(4) I assume your antibody is directly labelled (e.g. FITC). Try doing a
    double staining with CD3 (PE) on PBMC. You should get a diagonal staining
    pattern if the specificity is right (for the alpha/beta). You could then
    label isolated RBC the same way and see how they look like.
(5) Is the staining intensity of CD3 and your TCR mab similar on isolated PBL ?
(6) For further consideration could you please give details of the antibodies
    used?

Thanks in advance |

Martin

Dr. Martin R. Hadam
Medizinische Hochschule Hannover
Kinderklinik
D-30623 HANNOVER
TEL (49) 511 532 9009                E-MAIL HADAM@DHVMHH1.BITNET
FAX (49) 511 532 9016                       HADAM@VM.MHRZ.MH-HANNOVER.DE
Bundesrepublik Deutschland

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