From: Fumio Ike (fike@rkna50.riken.go.jp)
Date: Tue Oct 25 1994 - 02:58:26 EST
Hello, netters. I have several questions. Could you reply some of them, if possible ? 1. Can ELF from Molecular Probes be used in flow cytometry ? I want to stain intracellular proteins with AP-conjugated antibodies. Amplification of the signals using ELF is available ? Our EPICS ELITE ESP is equipped with He-Cd 325nm line. I think ELF would be excited by 325nm UV. Or, must I try to use Fast Red TR or something other substrates ? 2. Are TOTO-3 and TO-PRO-3 excited by Ar 488nm line ? I mean, when analysing cell surface antigens by FITC, PE and PerCP-labeled antibodies, can TOTO-3 and TO-PRO-3 be used as live/dead cell discriminators like PI using He-Ne 633 nm simultaneously ? 3. Is there anybody who uses AMCA ? Is this dye good for three or four color analysis ? Any comments and replies would be appreciated. Thank you. -------------- Fumio Ike, PhD. E-mail:fike@rkna50.riken.go.jp Animal and Cellular Systems Laboratory TEL:+81-48-462-1111(ext5613) The Institute of Physycal and Chemical Research (RIKEN) FAX:+81-48-462-4681
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