From: David Hedley (70740.1375@compuserve.com)
Date: Sat Sep 17 1994 - 12:13:18 EST
I'm starting to do a lot of work on the interrelations between reactive oxygen intermediates, redox status, and calcium regulation during cell killing by chemotherapy. Dichlorofluorescein is OK for detecting oxidative burst in neutrophils, but is too leaky to detect slow rates of generation of reactive oxygen by redox cycling agents. Also, a probe which picked up superoxide rather than hydrogen peroxide would be theoretically better. 1) Molecular Probes has a modified form of DCF which is stated to be better retained than DCF. Does anyone have any experience with it? Is it any good? (don't mean to be rude, Dick). 2) Dihydrorhodamine 123 is reputed to be more sensitive to superoxide. Does anyone have good evidence to support this, preferably published (grants panels and all that). 3) Does DCF respond to free radicals generally, or just to reduced forms of molecular oxygen? I'm particularly thinking about lipid peroxidation breakdown products. David Hedley Ontario Cancer Institute/Princess Margaret Hospital Toronto, Canada. P.S. I got some useful replies to my query about volume measurements. Will post a summary when all info available.
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