From: Rochelle A. Diamond (diamond@cco.caltech.edu)
Date: Fri Sep 09 1994 - 10:49:48 EST
Just thought I would add a contribution to the air bubble discussion. We (Caltech flow facility) have been purging our sheath fluid for years with Helium gas and a sparger. I learned to due this when I was working with HPLC many moons ago. It has worked great especially on those long sorting runs that last more than 8 hours. It doesn't seem to harm cell viability or function and we work with all kinds of cells- from mouse thymocytes to sea urchin coelomocytes. We let the helium sparge for about 15 minutes. Rochelle Diamond California Institute of Technology Flow Cytometry Facility Pasadena, CA 91125 (818)395-4947 diamond@cco.caltech.edu
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