From: Dave Coder (dave@nucleus.immunol.washington.edu)
Date: Mon Aug 29 1994 - 08:45:35 EST
On Fri, 26 Aug 1994, Thomas Knapp wrote: > > I would like to know how much UV laser power people are finding necessary for > calcium flux using "indo" staining. > > I am interested in knowing what UV power is being used on the Facstar Plus, > Facs Vantage as well as the Coulter Elite. On our FACStarPLUS we have a Coherent 90-5. It's an older laser design (not quite the controls as on current models), but very solid. We've used 150 mW of uv for indo-1; this power output draws 36.5A and has increased only 0.5A in three years (it's used in uv about 3 times a week). The FACStar is, of course, a jet-in-air system. A system with a cuvette will require less power. With Hoechst, we get good resolution (cv's on thymic cells can be less than 3%). Dave Coder University of Washington
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