From: Rehse, Mark (REHSEMA@cellpro.cellpro.com)
Date: Fri Aug 12 1994 - 10:37:37 EST
Dear Fellow Flow Experts, I am searching for a manufacturer of fluorescent beads. These beads must be 6-8 um in diameter, fluoresce under 488 nm excitation and emit from 525 to 675 nm for detection in the typical FL1, FL2 and FL3 configuration, and, here's the catch, have a fluorescence intensity similar to that of lymphocytes; about 1000-2000 MESF. Coulter Diagnostics sells Immunobrite beads as a kit which contains level II beads. These are perfect for my applications, however, they only sell these as a kit. To get one 5 mL bottle one must purchase the entire kit containing blanks plus 4 other fluorescence intensity standards. This is too expensive. If anyone knows of such a product could you please send the manufacturer's name and how they can be contacted. Many thanks in advance. Mark Rehse Rehsema@Cellpro.cellpro.com (206) 489-8850
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