From: Scott Cram (cram@telomere.lanl.gov)
Date: Mon Jun 06 1994 - 11:33:29 EST
Dear David, Many years ago (Exp. Cell Res. 80, 105-110, 1973) Dale Holm and I made the measurement you are asking about. Obviously the answer depends on the microspheres used and the instrument. At that time we were measuring CV's on lymphocytes of about 3-4% and microspheres were coming up with a CV of more like 3% or a little less. Our measurments indicated that about half the CV was due to true bead variability and half due to our flow cytometry measurement. More recently Dick Keller, Jim Jett, and John Martin (and others) at Los Alamos have been using their high sensitivity instrument and very small microspheres to repeat this measurement. With their system and the spheres used they were coming up with the same results - half and half. They can give you more details. Scott Cram Los Alamos
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST
