From: David Galbraith (dgalbrai@ccit.arizona.edu)
Date: Fri Jun 03 1994 - 16:55:12 EST
Fellow Cytometrists! I was wondering what the "absolute" CV of fluorescent microspheres might be? When we employ beads for alignment using flow cytometry, we measure a population CV (pulse height or integral) which presumably reflects a combination of the intrinsic error of the instrument as well as the error inherent to the beads themselves. Does anyone have any ideas as to the relative contributions of each of these factors to the measured CVs? What's the lowest CV anyone has obtained with fluorescent microspheres? I look forward to your responses. David Galbraith
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