autofluorescence

From: Mario Roederer (ROEDERER@Beadle.Stanford.EDU)
Date: Thu Mar 24 1994 - 16:08:56 EST 

We have approached the problem of autofluorescence from a different tack 
for a number of different applications:  compensation.  The fluorescence 
in a PE channel for unstained cells is reasonably well correlated with 
that in the FITC channel, especially for highly autofluorescent cells.  
Thus, by using the PE fluorescence to compensate FITC, one can 
essentially remove a majority of autofluorescence from the FITC channel. 
This improves sensitivity considerably (10x?) for measuring FITC 
fluorescences.  References for doing this real-time (Alberti, Parks, and 
Herzenberg) and in software (Roederer and Murphy) can be found in 
Cytometry in 1986.

mr

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