Yoyo in sea water

From: Daniel Vaulot (Daniel.Vaulot@univ-rennes1.fr)
Date: Thu Feb 17 1994 - 15:52:30 EST


 
 
 
Will anyone have experience with using YOYO, the DNA stain 
from Molecular Probe, in high ionic strength buffer?
We are working on marine phytoplankton and would like to stain 
cells directly in sea water.  Cells stain great in sea water diluted 10 
times in Qwater but when we decrease dilution, signal goes down 
and CV up drastically.  Is there anything we could to the sea watr 
to prevent this???
 
Thanks for your help.
 
Daniel Vaulot
Station Biologique BP74 
29682 Roscoff FRANCE 
Fax: 33 98 29 23 24
Vaulot@univ-rennes1.fr


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