From: vanburen%flovax.dnet@rocdec.roc.wayne.edu
Date: Mon Feb 14 1994 - 12:45:58 EST
Biomedical Engineer, BCC, asked about improving the separation between apoptotic cells and G0/G1 cells. We looked at apoptosis originally with PI, but have since switched to DAPI. In both cases, we were not interested in cell cycle information, so I just increased the HV gain such that the G0/G1 cells fell in the middle of the scale (512/1024). This seems to separate the populations a little better without adding too much noise. I haven't tried increasing the G0/G1 peak any higher than half-scale, since it was not necessary in our case, but it could be helpful. /\/\/\ Eric Van Buren, vanburen%flovax.dnet@rocdec.roc.wayne.edu \ \ \ Immunlogy & Microbiology \_^_/ Wayne State University
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:05 EST
