From: Centenary Institute (cenint04@angis.su.oz.au)
Date: Sun Oct 24 1993 - 21:36:13 EST
Some answers to your call over FloeCyt. The bad news is the data from FL2 & FL3 are gone! The good news is that there are ways of avoiding such surprises! The probable cause was the protocol settings left over from a previous run. The FACSCan/LYSYS combination is very versatile, and even a methodical person like me can't always remember all the details every time. Some rules & procedures I set up in our multi-user facility have been successful in getting the data saved as expected: 1) I recommend a routine calibration every day! We use BD CaliBrite, but others will do fine; The main point is that you have a known reference from which to compare. At very least, there should be a standard instrument settings file, but that will not help if/when your instrument performance changes. 2) ALWAYS quit and restart LYSYS between runs! ALWAYS! That way there are no surprises left over from the last run, (like a protocol with FL2 & FL3 turned off!) 3) Keep your own protocol, Reagent List, Instrument Settings etc. (if necessary, a set for each type of experiment) saved on a floppy &/or in your own directory. Then you can simply recall them and not have to remember to reset EVERYTHING! In the scenario I suggest, the FL2 data will appear on the screen while acquiring, but will not be saved if the protocol says "off" for that parameter. Good luck, and May the Flow be with You! Joseph Webster Centenary Institute Locked Bag No 6 Newtown NSW 2042 AUSTRALIA
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:25:59 EST
