color ccd's

From: Becky Bonner (f307@REX.RE.uokhsc.edu)
Date: Wed Oct 20 1993 - 09:55:44 EST

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Has anyone had any experience with the Optronics color ccd for
quantitative fluorescence?  (P.S. quantitative fluorescence is
defined as the amount of photons emitted from an object in
relation to the amount of probe/protein present....not percent of
cells or area of a certain color).  Is it linear?  How do you
handle color cross-talk with multiple fluorochromes?  How long
does it take to integrate a low level signal?  Are there any other
color ccd's that are good for quantitative fluorescence?  Ken
Castleman mentioned a camera at the meeting in Colorado but
was not at liberty to give brand names etc.  What camera was
that---any experience?  Are we still waiting for the technology?

Becky-Bonner@uokhsc.edu

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