From: Dave Coder (dave@nucleus.immunol.washington.edu)
Date: Wed Oct 20 1993 - 10:28:32 EST
One way has been to resuspend cells exposed to bacteria in a solution that contains
crystal violet which will quench fluorescein; ingested fluorescein-labeled bacteria
will stil fluoresce. Citations are in older literature 1982-1984. I can dig out some
if anyone is interested
Dave Coder
dcoder@u.washington.edu
Begin forwarded message:
Message-ID: <"70906191013991/3693 04061450*"@MHS>
To: list:;
Subject: Quenching of fluorescein
We want to measure phagocytosis of fluorescein-labeled bacter-
ia. To discriminate adhering from ingested particles we would
like to quench fluorescence of extracellular bacteria.
Is there any easy means besides use of a quenching anti-flou-
rescein antibody. A protocol or relevant citation of literature
would be gratefully acknowledged.
Please send response by E-mail. Thanks a lot.
Greetings from Frankfurt, Jo Betz.
------------------------------ End of forwarded message 1
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