From: Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
Date: Mon Sep 27 1993 - 17:53:29 EST
Has anyone had any trouble with non-linearity during DNA measurements on the FACScan? We have just suddenly found that, although the FL2-Height and FL3-Height signals show fluorescence intensity ratios at about exactly 2.0 for doublets and for G1/G2, the FL2-Area gives a ratio significantly greater than 2.0 for these peaks from the same samples. What are the reasons that the area of the fluorescence signal might not be linear with intensity? Why has this happened suddenly? Is this likely to be a fluidics problem or an electronic one? What can we do about it???? Thanks for any help anyone can offer. Alice Givan Alice.L.Givan@dartmouth.edu NCCC Flow Cytometry Laboratory/Department of Physiology Dartmouth Medical School Lebanon, New Hampshire 03756-0001 USA voice 603-650-7907 fax 603-650-6130
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:25:58 EST
