From: kharkins@iastate.edu
Date: Tue Sep 21 1993 - 18:28:17 EST
I have two questions, for which I hope to get some assistance from the network. First, we have a scientist who wishes to viably sort COS cells, which have been transfected with a plasmid, based upon the presence of a fluorescent ligand. Our facility policy has always followed the CDC NIH biosafety guideline. The ATCC catalog states that a BL2 (controlled aerosol) safety level should be followed with both COS 1 and COS 7 cell lines. Since jet in air sorting produces aerosol, our facility has normally not approved these projects. Are there instances where COS cells have been sorted (published/unpublished) and if so, is there a need to be concerned for the safety of the operator? My second question is unrelated to the first. We have a Coulter EPICS 750 series instrument and are currently collecting indo1 fluorescence (long and short) and ratio as listmode signals. We often have nice discernible shifts in the long & short histogram peaks, and the ratio in turn goes off scale. Is there an off-line software package (freeware or marketed) which is DOS compatible which will allow us to ratio the EPICS listmode short and long signals on a cell-by-cell basis? Any comments can be sent to me through the network or via the address below. Thank you in advance for your comments. Kristi R. Harkins kharkins@iastate.edu Iowa State University 1104 Molecular Biology Ames, IA 50011 (515) 294-2472 (place mail text here)
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