From: Christopher A. Worth (CAWORT01@ULKYVM.LOUISVILLE.EDU)
Date: Wed Aug 18 1993 - 14:55:44 EST
Biomedical Engineer, BCC Phone: (502)588-7191 I think your problem is that they dye is leaving your cells to get to an equili brium point with the system. When I was using PI-DNA analysis on a regular bas is, i found that the G0/G1 peak would drift right as the samplestarted running. What you may be experiencing is this same thing, however it is taking longer si nc you are using a different dye. The 7AD might just bind more tightly to the DNA and it takes longer for the eq point to be reached. My two cents, chris Avoid the Darkside, Use OS/2 2.1 These are my opinions, get your own. If I wanted your opinion I would squeeze one out of your computer.
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