From: Tom Mchugh (mchugh@jekyll.ucsf.EDU)
Date: Wed May 19 1993 - 14:52:33 EST
We use the CD Chex cells routinely for lymphocyte antigens. The standard antigens (2,3,4,5,7,8,19,20, etc.) seem to work fine. We have tried CD 13, 14 and 33 and they have worked. I have not noticed that the background fluorescence is very high but we have not done this much and I will go back and look. We have seen some unusual staining with these cells, for example some vendors anti-CD 10 stain the cells and a target value is listed for CD 10. BD's anti-CD 10 does not stain the cells and CD says the staining for CD 10 is non-specific yet reproducible. Some other less commonly used antigens also stain to different levels of acceptance.
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