From: kukuruga%kasle1.dnet@rocdec.roc.wayne.edu
Date: Thu Apr 29 1993 - 15:45:53 EST
If you are having problems "as the samples are being run", its seems more likely you problem lies in the instrument, unless you have reason to believe otherwise....perhaps more details on your protocol will shed more light. Points to ponder... 1) Bird RBCs may be nucleated (hence the "CEN" DNA standard); this may effect your overall analysis approach. I don't know about lizards, but considering the link, this may be true for them also. 2) One might be well-advised to consider the existance of naturally-occuring DNA configurational anomolies (eg. polyploidization, as in fishes)...these may also be sources of variability. 3) DNA content staining protocols can be extremely cell-type, and animal-source specific. Therefore, procedures which work well on mammalian cells may prove disastrous for your samples. MAK.
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