From: Dave Coder (dave@nucleus.immunol.washington.edu)
Date: Tue Apr 20 1993 - 19:49:06 EST
Data acquisition from cytometers that is bidirectional (as in the FACScan, FACStar, Elite, etc.) requires that both data from the cytometer be collected (most likely to disk), and instrument operation values be sent from the computer to the cytometer. This kind of instrument control from the computer is reasonably tricky. Cytomation is the only company to offer commercial solutions. The more simple problem of data acquisition can be accomplished far more easily, but I don't know of any solutions other than that from Cytomation mentioned above. For older instruments, FACS440 and several predecessors, there are a variety of acquisition solutions that have been implemented. These include various DEC-based systems (Electric Desk, ACQ at NIH, and others) and the PC-based acquisition system that I put together (an abstract is in the Cytometry supplement for the ISAC meeting in Bergen). The system allows 4-parameter acquisition on a PC and can run effectively up to 10,000 cells per second. It can run quite nicely in DESQView and probably does fine as a non-Windows application. Dave Coder Dept. Immunology University of Washington Internet: dcoder@u.washington.edu
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