From: auger@ux1.cso.uiuc.edu
Date: Wed Feb 24 1993 - 16:38:53 EST
Can anyone give me references to working with cell surface antigens (i.e. CD4/CD8) on frozen/thawed lymphocytes? Is it possible without introducing artifact? What kind of conditions (freezing/thawing) must be met to make the procedure possible? I imagine that there have been some published reports on the feasibility of freezing isolated lymphocytes and then staining them with various monoclonals, but quite frankly, if someone can send me their advice, it saves me from going to the library! I would appreciate any and all comments. Thanks. ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Julie A. Auger, Director 217-244-0559 voice Flow Cytometry Facility 217-244-0466 fax University of Illinois at Urbana-Champaign auger@ux1.cso.uiuc.edu 231 PABL, 1201 W. Gregory, Urbana, IL 61801 ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
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