From: Gary Durack (gary@flowcyt.cyto.purdue.edu)
Date: Fri Feb 12 1993 - 03:49:08 EST
I would appreciate hearing from anyone that has done bivariate flow karyotyping of chromosomes using Chromomycin and Hoechst with either an EPICS Elite or EPICS 752. In particular I am interested in exactly how the "gated amp" trigger should be set up to acheive the best results. FALS seems to be un unreliable trigger source for this application and there will always be fluorescent emmision from both the UV and 458nm beam at nearly the same wavelengths. The gate trigger will only work properly on a "clean" signal that is unique to the top beam. So my question is : what is the best signal to use for this application? gary durack Purdue University Cytometry Laboratories fax (317) 494-0517 phone (317) 494-8638
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