Gérald GREGORI
Laboratoire de Microbiologie, de Géochimie et d 'Ecologie Marines
LMGEM - CNRS UMR 6117
Campus de Luminy - Bât TPR1 - Entrée G - Case 901
13288 Marseille cedex 9
Tél. : (+33) 4.91.82.91.14
Fax : (+33) 4.91.82.96.41

gregori@com.univ-mrs.fr


PhD of the "Université de la Méditerranée"

mention "Science of the Marine Environment"




Scientific field:
Functional Microbiology



French version French version
Who I am...
CV  (short)

TECHNICAL AND ANALYICAL SKILLS

My works during the PhD
SUMMARY
MANUSCRIPT (pdf format 5.2 MB)

My field of research

My "favorite" scientific tools
FLOW CYTOMETRY
MICROSCOPY


Life in the USA...


Useful links

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 




Curriculum vitae (short version)
31 years old, married, one child.

Post-doctoral Research Assistant at Purdue Unversity (Indiana, USA) in the Purdue University Cytometry Laboratories (scientific director : Professor J. Paul Robinson).

PhD of oceanography at the "Université de la Méditerranée" (Aix-Marseille II, France), public defense on 12/12/2001 (highest distinction : Mention tres honorable avec les félicitations du jury)

Research field
Aquatic microbial ecology - Mineralisation of the organic matter
Keywords : ultraplankton, respiration, bacterial viability , cytometry.


Professional skills:

- Sampling and analyses of natural samples

- Flow cytometric and microscopic analyses of procaryotic and eucaryotic cells (nucleic acids, photosynthetic pigments, membrane potential, enzymatic activities, cell viability, etc.)

- Handling of fluorescent dyes for flow cytometric and microscopic analyses

- Handling of the following flow cytometers:
     - FACSort and FACScalibur (Becton Dickinson),
     - Cytomics FC 500, Epics XL-MCL, Epics Elite (trieur), Altra (sorter) (Coulter)
     - Cytoron Absolute (Ortho-Diagnostic Systems)
     - Agilent 2100 Bioanalyzer (Agilent)

- Handling of classical, epifluorescent and confocal microscopes

- Data analyses and presentations (talk and/or poster)

- Scientific drafting


Scientific publications

J. P. Robinson, B. Rajwa, G. Gregori, J. Jones, V. Patsekin (2005). Multispectral cytometry of single bio-particles using a 32-channel detector. Proc. SPIE (5692) : 359-365. Advanced Biomedical and Clinical Diagnostic Systems III; Tuan Vo-Dinh, Warren S. Grundfest M.D., David A. Benaron M.D., Gerald E. Cohn; Eds.

Zhou L., G. Gregori, J. M. Blackman, J. P. Robinson and B. L. Wanner (2005) Stochastic activation of the response regulator PhoB by noncognate histidine kinases. In: Yearbook Bioinformatics 2004, R. Hofestädt (ed.), pp. 25-38. Magdeburg: IMBio, Informationsmanagement in der Biotechnologie e.V.

Wang WH, Gregori G, Hullinger RL, Andrisani OM (2004). Sustained activation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase pathways by hepatitis B virus X protein mediates apoptosis via induction of Fas/FasL and tumor necrosis factor (TNF) receptor 1/TNF-alpha expression. Mol Cell Biol. 24(23):10352-65.

G. Grégori, M. Denis, D. Lefèvre, J.C. Romano (2003). Viabilité des bactéries hétérotrophes dans la baie de Marseille. Comptes Rendus Biologie. 326 : 739-750.

B. Desnues, C. Cuny, G. Grégori, S. Dukan, H. Aguilaniu, T. Nystrom (2003). Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells. EMBO Reports 4 (4): 400-404.

G. Grégori, M. Denis, D. Lefèvre, B. Becker (2002). A flow cytometric approach to assess phytoplankton respiration. Methods in Cell Science 24: 99-106.

G. Grégori, M. Denis, S. Sgorbati, S. Citterio (2003). Resolution of viable and compromised-membrane bacteria in aquatic environments by flow cytometry. Current Protocols in Cytometry (J.P. Robinson, Z. Darzynkiewicz, W.C. Hyun, A. Orfao, P.S.and Rabinovitch, eds.) pp 11.15.1-11.15.7. J.Wiley & Sons, inc., New York.

G.Grégori, A. Colosimo, M. Denis (2001). Phytoplankton group dynamics in the bay of Marseilles during a 2-year survey based on analytical flow cytometry. Cytometry 44: 247-256.

G.Grégori, S. Citterio, A. Ghiani, M. Labra, S. Sgorbati, S. Brown, M. Denis (2001) Resolution of viable and membrane compromised bacteria in fresh and marine waters based on analytical flow cytometry and nucleic acid double staining. Applied and Environmental Microbiology 67: 4662-4670.

Christaki U., Giannakourou A., Van Wambeke F. and Grégori G. (2001) Nanoflagellate predation on auto- and heterotrophic picoplankton in the oligotrophic Mediterranean Sea. Journal of Plankton Research 23: 1297-1310.

Grégori G. (2001) Ultraphytoplankton in the Bay of Marseilles : time series, cell respiration and bacterial viability. Thesis, Université de la Méditerranée, Marseilles, France.

Grégori G. (2002) Ultraphytoplankton in the Bay of Marseilles : time series, cell respiration and bacterial viability. JRO 27(2): 109-111.

U. Christaki, A. Giannakourou, F. Van Wambeke, G. Gregori (2000). Picoplankton and nanoplankton coupling in the euphotic zone of the Mediterranean Sea. CIESM Workshop Series 11 : 25-26

 

Miscellaneous

5 Invention Record Disclosures with patent in process (number P-03096, P-03073, P-03150, P-03151, P-03152)
     
More than 20 participations (oral communications and posters) in national and international congress (Prize for the best student oral presentation in 2001 ; Prize for the best student poster in 1997)

2 oceanographic cruises (Chief of Mission for the RASUR cruise)

Qualified in the  67th section (Biology of populations and ecology) of the National Conseil of the Universities (CNU)

Membre of the International Society for Analytical Cytology (ISAC)

Membre of the American Society of Plant Biologists (ASPB)

1997-2002 Lectures and practical sessions from level DEUG to Maitrise in the "Université de la Méditerranée"

2003 Teaching during the workshop "Cytometry Applications in Cellular Biology and Medicine" (Minho University, Portugal), July 28th - August 1st

2004 Lecture and practical sessions in the workshop "The Role of Flow Cytometry in Marine Biodiversity and Ecosystem Functioning". A course for post-graduates and Early Stage Researchers Sponsored by the EU Framework 6 Network of Excellence in Marine Biodiversity and Ecosystem Functioning (MARBEF) and the Stazione Zoologica "Anton Dohrn", Naples, Italy. With the support of the Italian Society of Cytometry (GIC) 3-6 November 2004.

2004 Teaching lectures and practical session at Purdue University (USA)

Reviewer for the journals Cytometry, Clinical Cytometry and Aquatic Microbial Ecology

1998-2000 « Programme d’Actions Intégrées » GALILEE (French-Italian cooperation program): Development of a method for the monitoring of Mediterranean coastal waters by using analytical flow cytometry.  PIs : M. Denis (Marseilles, France) and S. Sgorbati (Milan, Italie).


1998-2000 « Programme d’Actions Intégrées » PICASSO (French-Spanish cooperation program): Dynamics and activity of microbial assemblages in Mediterranean coastal waters. PIs: M. Denis (Marseilles, France) and  D. Vaquè (Barcelone, Espagne).

1998-2000 Elected student member to the Administration Council of the Centre d’Océanologie de Marseilles (OSU)


2001 Co-organisation of the "Sciences de l'Environnement" doctoral school congress. Member of the Scientific Committee.

2003 Coorganisation of the PUCL Openhouse

Since 2003 Codevelopment of the PUCL WebSite



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