|
|
TECHNICAL AND ANALYTICAL SKILLS
Sampling
and preparation of natural aquatic samples
- Sampling and analyses of natural freshwater and marine samples
- Filtrations, fixation (Ethanol, Paraformaldéhyde) of natural
samples
- Measure of O2 concentration by photometry (Winkler method)
- Microbiological classical methods: Bacterial cultures and monospecific
cultures of autotrophic aquatic microorganisms (Chlorophyceae, Bacylariophyceae)
Flow Cytometry
- Technical
Skills:
- Handling of benchtop cytometers
(CYTORON ABSOLUTE (Ortho Diagnostic Systems), CYTOMICS FC500 et EPICS
XL-MCL (Beckman-Coulter), FACScalibur (Becton Dickinson),Agilent 2100
Bioanalyzer (Agilent Technologies)) ;
- Handling of sorters (Epics Elite and Altra (Beckman-Coulter)) ;
- Installation, alignment of air and water-cooled lasers;
- Installation of fiber optic
- Optical alignment of one or several lasers (colinearity);
- Development of new technologies at the Purdue University Cytometry
Laboratories (USA)
-
Analytical Skills :
- Measurement of cellular parameters
of auto- and heterotrophic prokaryotes and eukaryotes (nucleic acids,
membrane potentiels, membrane integrity, photosynthetic pigments,
size and structure, cell cycle, etc.) ;
- Sorting.
Static
Cytometry
- Optical, epifluorescent, confocal
and multiphoton microscopy
- Image analysis
- Development of new material at the Purdue University Cytometry Laboratories
(USA)
Cell Staining
Handling of fluorescent
dyes (fluorochromes) and monoclonal antibodies for flow cytometry
and microscopy;
Respirometry
Technics
Handling of the High Resolution Oxygraph
(OROBOROS)
Data
analysis
- Data analysis of data from flow cytometry and micrsocopy
- Artificial Neural Networks (Kohonen's Self Organizing Maps)
|
