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Flow Cytometry

Epics XL-MCL (Coulter)

The Coulter XL-MCL is equipped with a 488nm laser and is able to analyze three fluorescences and two scatter signals simultaneously. Its Multiple Carousel Loader (MCL) makes the Epics XL-MCL capable of throughputs of up to 100 samples per hour.

 

Lasers

- Air-cooled Argon ion laser, 488nm (20mW output)

 

Detectors

- Forward Scatter detector is a photodiode

- Right Scatter is a PMT

- Four photomultipliers with spectral sensitivity from 185nm-900nm collect the fluorescence signals centered approximatively at:

- FL2 = 525 nm
- FL3 = 575 nm
- FL4 = 625 nm

Flow Rates

- A continuous pressure is applied to the sample tube based on user selected flow rates.


- Three flow rates are available:

- Low
- Medium
- High

 

Sample Handling

- Glass or plastic tubes (12x75 mm)

- Single-tube sampling mode

- Multi Carousel Loader (up to 32 tubes)