Live Cell Imaging Applications in Confocal Microscopy

4/3/2000


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Table of Contents

Live Cell Imaging Applications in Confocal Microscopy

Applications

Applications

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Example of DIC and Fluorescnece

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Measurement of DNA

PI - Cell Viability

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Specific Organelle Probes

Organelle Function

Calcium Related Applications

Probes for Ions

Ionic Flux Determinations

Calcium Flux

Oxidative Reactions

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Hydroethidine

Macrovascular Endothelial Cells in Culture

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H2O2 stimulation and DCF & EB loading in Rat Pulmonary Artery Endothelial Cells

pH Sensitive Indicators

Exotic Applications of Confocal Microscopy

“Caged” Photoactivatable Probes

Release of “Caged” Compounds

Release of Caged Compounds

Membrane Polarization

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FRAP

4D confocal microscopy

4D Imaging

Imaging 3D ECM structures

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Thick Tissue - Bone and Cartilage

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Lecture Summary

Author: J. Paul Robinson,
Purdue University Cytometry Laboratories

Home Page: //flowcyt/educate.htm

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