Sample Preparation of Confocal Microscopy

4/3/2000


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Table of Contents

Sample Preparation of Confocal Microscopy

Characteristics of Fixatives

Ideal Fixative

Chemical Fixation

Coagulating Fixatives

Coagulating Fixatives

Crosslinking Fixatives

Crosslinking Fixatives

Glutaraldehyde

Glutaraldehyde

Formaldehyde

Ethelene glycol-bis-succinimidyl succinate (EGS)

Fixation and preparation of tissue

Fixation Protocol pH-shift/Formaldehyde

Method

Fluorescence Labeling

Examples of Fluorescent labels

Rhodamine 123

PPT Slide

PPT Slide

PPT Slide

Test Specimen

Test Specimen - Onion

Test images

Summary

 Author: J. Paul Robinson,
Purdue University Cytometry Laboratories

Home Page: //flowcyt/educate.htm

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