Nikon : Eclipse E800 : EPI-FL Filters

		Eclipse E800 : EPI-FL Filters Eclipse E800

Epi-Fluorescence Filters

Photomicrographic Equipment Nikon offers the following filter combinations to meet almost every application.

Filter Characteristics

	Filters	Wavelengths	Characteristics	Applications
U V	UV-2A	EX330-380 DM400 BA420	Standard filter block for UV	DAPI Hoechst 33258/33342 AMCA Cascade Blue*
	UV-2E/C* (DAPI)	EX 340-380 DM 400 BA 435-485	For DAPI, cutting off FITC (green) and TRITC (red) Soft-coated type for high signal/noise Band-Pass Barrier Filter used to cut off green and red
	UV-1A	EX 365/10 DM 400 BA 400	Narrow band pass -- only 365nm (i line) of Mercury spectrum used Narrow band pass minimizes auto-fluorescence and photo-bleaching
	UV-2B	EX 330-380 DM 400 BA 435	Darker background than UV-2A
V	V-2A	EX 380-420 DM 430 BA 450	Standard filter block for V	Catecholamine Serotonin Tetracycline
	V-1A	EX 405/10 DM 430 BA 435	Narrow band pass - only 450 nm (h line) of Mercury spectrum used Narrow band pass minimizes auto-fluorescence and photo-bleaching
	V-2B	EX 380-420 DM 430 BA 460	Darker background than V-2A
B V	BV-2A	EX 400-440 DM 455 BA 470	Standard filter block for BV	Quinacrine Quinacrine Mustard (QM) Thioflavine S Acriflavine
	BV-1A	EX 435/10 EM 455 BA 470	Narrow band pass - only 435 nm (g line) of Mercury spectrum used Narrow band pass minimizes auto-fluorescence and photo-bleaching
	BV-2B	EX 400-440 DM 455 BA 480	Darker background than BV-2A
B	B-3A	EX 420-490 DM 505 BA 520	Wide band pass - recommended for halogen illumination only	FITC Acridine Orange Auramine O Coriphosphine O Bodipy® Fluo-3 DIO
	B-2A	EX 450-490 DM 505 BA 520	Standard filter block for B For FITC + Counter-stain (TRITC, PI)
	B-2E/C* (FITC)	EX 465-495 DM 505 BA 515-555	Soft coated type for high signal/noise For FITC (green), cutting off Rhodamine red Band-pass Barrier Filter used to cut off red
	B-1A	EX 470-490 DM 505 BA 520	Narrower excitation range than B-2A FITC + Counter-stain (TRITC, PI)
	B-2E	EX 450-490 DM 505 BA 520-560	Similar to FITC For FITC (green), cutting off Rhodamine red Band-Pass Barrier Filter used to cut off red
	B-1E	EX 470-490 DM 505 BA 520-560	Hard coated type for FITC Band-Pass Barrier Filter used to cut off red
G	G-2A	EX 510-560 DM 575 BA 590	Standard filter block for G	TRITC Rhodamine B200 Propidium iodide R-Phycoerythrin B-Phycoerythrin Dil Ethidium Bromide
	G-1A	EX 546/10 DM 575 BA 580	Narrow band pass - only 546nm (e line) of Mercury spectrum used Narrow band pass minimizes auto-fluorescence and photo-bleaching
	G-2E/C* (TRITC)	EX 540/25 DM 565 BA 605/55	For TRITC (Rhodamine) Soft coated type for high signal/noise Band-Pass Filter used to cut off reds above 643nm
	G-1B	EX 546/10 DM 575 BA 590	Narrow band pass - only 546nm (e line) of Mercury spectrum used Narrow band pass minimizes auto-fluorescence and photo-bleaching
	G-2B	EX 510-560 DM 575 BA 610	610nm barrier provides darker background and deep red emission
Y	Y-2E/C* (Texas Red)	EX 540-580 DM 595 BA 600-660	For Texas Red® Soft coated type for high signal/noise Band-Pass Barrier Filter used to cut off reds above 660nm	Texas Red®

Multi-Band Filters*

Filters	Abbreviations	Reagents
Dual	F-R	FITC Rhodamine
	F-T	FITC Texas Red
	D-F	DAPI FITC
Triple	D-F-R	DAPI FITC Rhodamine
	D-F-T	DAPI FITC Texas Red

GFP Filters*

Filters	Wavelengths	Characteristics	Applications
GFP (R) -LP	EX 480/40 DM 505 BA 510	Long-pass type GFP red shifted mutants	GFP
GFP (R) -BP	EX 480/40 DX 505 BA 533/50	Band-pass type for GFP red shifted mutants	GFP