|
Andrea Cossarizza, Stefano Salvioli Specimen: HL-60 cells |
|
|
Description: Confocal Analysis of HL-60 cells treated with staurosporin (STS) for 4 hours and stained with FITC Annexin-V (Bender MedSystems, Austria), detecting cells undergoing apoptosis and MitoTracker Red CMXRos (Molecular Probes, OR, USA), for mitochondrial membrane potential.
|
)
Click to enlarge. )
Click to enlarge. |
|
Description: Note that after STS treatment an apoptotic cell (positive to Annexin-V) still maintains a high mitochondrial fluorescence (arrow, A), indicating that membrane potential is not disrupted, while another has a low potential (arrow, B).
|
)
Click to enlarge. )
Click to enlarge. |
|
Description: The third set of pictures shows a magnification of cells (one healthy, one apoptotic) labelled with the above mentioned probes.
|
)
Click to enlarge. )
Click to enlarge. )
Click to enlarge. )
Click to enlarge. |