Simple example of Data
"Acquisition" & one instance of how a "clog" may
appear:
HELP INDEX
-
Please
open the two files "BLOCK" and "NOBLOCK" from the Event Source
selection window and display a dotplot of FSC-H vs
SSC-H.
- Now,
select the Acquire menu, and select the "float" option. A floating
window will appear to enabling the starting, stopping and
restarting of the active data display which displays data in an
identical fashion to that which occurs on a flow
cytometer.
- Click
the "Acquire" button to begin "acquiring" data. Note that a pulse
height display appears in the "Float" window which is generated
from events being displayed. (NOTE: High, Medium and Low default
flow rate buttons are currently disabled)
- Now
watch the dotplots closely, noting how the Forward and Side
Scattered light patterns change when a partial clog or blockage in
the sample line or nozzle, affects in the data stored in the file
"BLOCK".
This
is an example of how Facsimile can simulate things which occur on
a real instrument, and teach new users how to recognise when a
partial blockage has occurred. The flow rate number of dots
displayed and so on can all be adjusting to suit the user (see
View menu: Modifying displays, "flow rates" etc:
below).