Facsimile: Regions and Gates Help Topic

Help Index

Regions can be created using the following procedure.

  1. Create a dotplot either by opening a new file or selecting "Plots", "Dotplot".
  2. Right click (Macintosh APPLE key right click) in the dotplot and select "Region", "Create new".
  3. Click around the population of cells of interest, closing the region by clicking in the small black square.

Regions can not be moved. If the region is not in the correct position, remove the region and redraw it.
Regions can be removed using the following procedure.

  1. Right click (Macintosh APPLE key right click) in the dotplot containing the region you wish to remove.
  2. Select "Region", choose the region number you want to remove, for example "R0", select "Remove".

Gates can be created using the following procedure. Create regions (see above).

  1. Windows and Unix OS:
    1. Click on the region you want to use for gating.
    2. Drag the region and drop it onto the plot you want gated. This appies AND logic to the gate.
    3. To use OR logic, Ctrl drag the next region onto the plot. Note you need to AND logically gate the first population.
  2. Macintosh OS X (or Windows and Unix OS):
    1. Right click on the plot you wish to apply the region as a gate too.
    2. Select "Gate","Add","AND", for example "R0".
    3. To use OR logic, Select "Gate","Add","OR", for example "R1". Note you need to AND logically gate the first population.

Additional points to note:
Histogram overlays are best gated adding all the plots to the desired Histogram Overlay window then applying the gate.
Colours associated with regions can be set under the "View", "Options" and "Display" tab.
Histogram regions are not yet implemented..

Last updated 12th August, 2003 G.W.Osborne