Simple example of Data "Acquisition" & one instance of how a "clog" may appear:

HELP INDEX

1. Please open the two files "BLOCK" and "NOBLOCK" from the Event Source selection window and display a dotplot of FSC-H vs SSC-H.

2. Now, select the Acquire menu, and select the "float" option. A floating window will appear to enabling the starting, stopping and restarting of the active data display which displays data in an identical fashion to that which occurs on a flow cytometer.
3. Click the "Acquire" button to begin "acquiring" data. Note that a pulse height display appears in the "Float" window which is generated from events being displayed. (NOTE: High, Medium and Low default flow rate buttons are currently disabled)
   
4. Now watch the dotplots closely, noting how the Forward and Side Scattered light patterns change when a partial clog or blockage in the sample line or nozzle, affects in the data stored in the file "BLOCK".

This is an example of how Facsimile can simulate things which occur on a real instrument, and teach new users how to recognise when a partial blockage has occurred. The flow rate number of dots displayed and so on can all be adjusting to suit the user (see View menu: Modifying displays, "flow rates" etc: below).