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How to use VIEWDATA?
The purpose of this program is to inspect Cytosense data files and covert
files to data for analysis with CytoWave, WinMDI and spreadsheets such
as EXCEL
Instrument: The serial number or nickname of the instrument used to aquire
the data.
File Selection :
Use the boxes under RAW DATA SELECTION to select the raw data files. Note:
Cytosense data files are organised in "clusters", One "header"
file and three to five data files. The filenames box shows only the names
of the "header" files.
While navigating through the directories it looks for a file named "Instrumentdata.txt"
that contains how the Cytosense instrument is configured. If it does not
find that file then it will show DEFAULT as instrument and use nominal
values.
"Kind of particle" selection :
Below the filenames box there are 5 boxes to choose the type of fluorescence.
If you choose there BEFORE clicking on the file name then only the chosen
type of particles will be displayed. E.G. if you want to suppress the
non fluorescing "dead" particles such as sediment then uncheck
the box "No Fluorescence".
Dotplot :
The program makes a plot of integrated forward light scatter vs particle
length. Each particle appears as a circle , the diameter of the circle
is an indicationof the strength of fluorescence , the color indicates
the dominant fluorescence wavelength. Non fluorescent particles are shown
as small black circles.
Count box :
Four lines below the dotplot show particle counts within the selected
box. Initially this is the whole dotplot, unless you have clicked the
button " Keep Box ". Then the last selected box stays valid
when you select a new file. In this way you can make a report of counts
in one area over multiple files.
Selecting particles to count and view "pulse shapes" :
Move the mouse in the dotplot , press the left button where you want
the first corner of the selection rectangle and release at the other corner.
A new line will appear in the top of the couts box with a count of particles
within the drawn rectangle. Note: you can scroll through the count lines,
but they are written "from bottom to top", the last result is
on the top line.
Viewing "pulse shapes" :
Once a box is selected, clicking in the upper left picture shows a
graph of Forward scatter, Sideward scatter and fluorescence vs length
of the particle. The displayed particle turns blue in the dotplot. Particles
are shown in the order of arrival until all particles in the box have
been shown. NOTE: this is raw data as measured. Since the laser beam is
about 5 microns wide, small particles will appear substantially longer
than the indicated length where the beamwidth is accounted for. The box
can be dragged and reshaped.
Print : Clicking this button sends the picture of the box to the printer.
Save : Lets you make a list of the data of the displayed particle.
Saving results:
Click on "Save result files" to get a dialog box to produce
and save results as one of the following formats:
- name.CPF : raw data listmode file for the CytoWAVE analysis program.
- name.FCS : integrated data listmode file in standard FCS3.0 format
for programs such as WinMDI
- name.LST: integrated data listmode file as text, usable in spreadsheet
programs such as EXCEL.
- name.DST: distributuon in 10% lengthbins as on top of the dotplot,
as textfile.
- name.TXT: all raw data as text file. NOTE: this file is large, up
to several MByte.
Saving the box selection results:
Click the "Save" button to save the box counts displayed as
*B.TXT file. This file can be imported in spreadsheets such as EXCEL.
Note: the box counts text is "infinitely long", to clear click
on the clear button.
Print Screen:
Clicking on the "Print Screen" sends the comlete screen to the
printer. Alternatively you can press "PrtSc" button on the keyboard
to copy the screen as bitmap to the clipboard and edit later in a graphics
program like "PAINT" .
Note: you can edit this text , "Viewdata.TXT",
at will.
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