Happy Holidays to all my fellow 'flow'ers! I have a question regarding the CAP lymphocyte immunophenotyping surveys for clinical flow labs. We have difficulties setting the gates appropriately due to the quite altered scatter pattern of the preserved survey specimens. We also have issues with low white cell counts. Does anyone else have these or other problems? Do you have any suggestions for processing or analyzing that we could try? Thanks for your input, Melanie O'Donahue, MT, ASCP Mayo Clinic Scottsdale Flow Cytometry Lead Technologist Odonahue.Melanie@mayo.edu 480-301-8028
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