Hello, I am posting the summay of the info I received, regarding the search of the whole blood preservatives for sorting and mRNA works. Thank you for those who responded to my inquiry. Although there isn't any perfect solution yet, I hope the info here will provide leads in the future. ======== Currently two such products exist, one by Ambion and the other by Qiagen, both work off the same principal being that they neutralize Rnases and all other proteins in the blood. I just was informed about eh Qiagen product by a rep last week, so its new to the market. Qiagens product is one where the blood is directly drawn into the collection tube along with the RNA degradation inhibitor already present so real time RNA levels are qmaintained. PAXgene Blood RNA System. You may wish to visit the site at http://www.qiagen.com/catalog/chapter_06/paxbloodrnasystem.asp It seems to be very suitable for this application. However, it will lyse the cells according to the conversation with the technical support people. Thus, sorting of some subsets may not be possible. It is good for maintaining mRNA level in the whole blood. I can not locate the product by Ambion. =========== I don't have a certain answer for your mRNA question, but I advise trying Proclin before you give up. It's a metabolic inhibitor we used (back in the days when I worked for CompuCyte Corp.) to use to stabilize whole blood for clinical trials looking at surface markers. You may still get some degradation--RNase is EVERYWHERE--but at least it'll shut down transcriptional changes triggered by activation in transport.... Although through web serach, I found out that ProClin was manufactured by Rohm and Haas. I can not find the product on the web (www.RohmHass.com) any more. However, Sigma carries ProClin 150/200/300. Any publication using ProClin in this application will certainly help. =========== My experience is that there are good perservatives that stabilize the blood, but they tend to cross link mRNA and prevent analysis. The samples work great for flow. Proceed with caution. ========= Fang-Yao Stephen Hou, Ph.D. Flow Cytometry Specialist UC-Irvine
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