Hi all, I have a question concerning the use of Alexa 350, bound to a monoclonal Ab. On a flourescence microscope, we could detect stained cells. However, on the flow cytometer (MoFlo with a Coherent I90C UV Laser), I could not detect a signal. Molecular Probes suggested, that the energy (100mW) of the Laser would bleach the flourochrome and therefore no signal is detected. Is this possible? Or is the Argon Laser bleaching the compound? The system has two lasers, 488 nm Argon Laser at 100mW on position 1 and the UV Laser on position 3 (refering to the pinholes). The Filters for the detection of Alexa 350 was 450/65 of a dichroic 510LP mirror. Thanks for any hints. Merry Christmas and Happy New Year! Ruedi Dr. Ruedi K Braun Ottawa Health Research Institute 501 Smyth Rd Ottawa, ON K1H 8L6 Canada phone: (613) 737-8899 ext 73916 e-mail: rbraun@ohri.ca
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