James, In order to reduce salt buildup on slides when sorting rare events I have on a FACStar+ routinely sorted using PBS diluted 1:300 to 1:500. 1:1000 dilution was (on our instrument) also possible , but somewhat unreliable. You would propably have to adjust the droplet charge, pulse shape, and phase significantly in comparision to sorting with PBS. Jan Grawé >Hello, > >We have a client who is trying to sort a fresh water protozoan that >expresses GFP. They are very worried about stressing the cells. They >have found that stressing these cells causes them to release their >GFP and thus show up negative. I was wondering if anybody has >determined the minimum concentration of salt that can be used in the >sheath fluid that will still hold a charge. Presently we use a 1X >PBS solution consisting of NaCl, KCl, Na2HPO4 7H2O, and KH2PO4 > > > Thanx in advance, Marv -- -------------------------------- Jan Grawé Cellanalyslab Rudbecklaboratoriet/C5 Uppsala Universitet Dag Hammarskjölds väg 20 75185 Uppsala tel: +(0)18-4714656 mobil:+(0)70-2577874 fax: +(0)18-552739 epost: jan.grawe@rudbeck.uu.se http://www.rudbeck.uu.se/cellanalys
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