Importance of this forum/Use of FL1, FL2/FACS Question

From: Reed, Doug S Dr USAMRIID (Doug.Reed@DET.AMEDD.ARMY.MIL)
Date: Wed Dec 19 2001 - 15:23:01 EST


I think in the spirit of the season and the aftermath of 9-11 we should drop this
thread now, it seems to be spiraling out of control.

I will say this - Paul, you are right that for publication people should not use FL1,
FL2, FL3. How much detail is provided is an appropriate thing to consider, I myself
place it in the context of what the paper is discussing. If it is a paper advancing
the art of flow cytometry, it should have as much detail as possible. For a paper
discussing, for example, phenotypic analysis of lymphocytes I would think that saying
CD4-FITC should be more than sufficient.

For this forum, however - in my humble opinion I think you are dead wrong. In the 6
years or so I've been a member this is and always has been a safe haven for new flow
users to find out what they need to know without being attacked. Correcting their flow
"grammar" is appropriate, as is pointing people to the archives to answer a question. But
the manner in which this email was answered in this case can hardly be called polite. To
be honest I was absolutely shocked at what can only be called a rant - it goes totally
against the grain of this forum and unlike anything I've seen while I've been a member.

Sincerely,
Doug Reed

Douglas S. Reed, Ph.D.
Principal Investigator
Respiratory and Mucosal Immunity
Department of Aerobiology & Product Evaluation
Division of Toxinology & Aerobiology
U.S. Army Medical Research Institute of Infectious Diseases
1425 Porter St. Ft. Detrick
Frederick, MD 21702-5011
301-619-6728
301-619-6911 (Fax)
doug.reed@det.amedd.army.mil



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